Rear-polarized Wnt5a-receptor-actin-myosin-polarity (WRAMP) structures promote the speed and persistence of directional cell migration

Supplemental Materials

This article contains the following supporting material:

• Supplemental Materials
• Movie S1 - WRAMP structures form dynamically and are followed temporally by membrane retraction.Three WM239a cells expressing MCAM-GFP treated with Wnt5a, imaged for 3h. Scale bar = 20 μm; time stamp = h:min.
• Movie S2 - MCAM and F-actin movement in WM239a cell. Time series corresponding to Figure 2A.
• Movie S3 - MCAM and F-actin movement in A375 cell. Time series corresponding to Figure 2B.
• Movie S4 - MCAM and myosin IIB movement in WM239a cell. Time series corresponding to Figure 2C.
• Movie S5 - MCAM and myosin IIB movement in A375 cell. Time series corresponding to Figure 2D.
• Movie S6 - Control for WRAMP formation with mCherry and MCAM-GFP in WM239a cells. Time series corresponding to Supplemental Figure S1 A.
• Movie S7 - Control for WRAMP formation with mCherry and MCAM-GFP in A375 cells. Time series corresponding to Supplemental Figure S1 B.
• Movie S8 - WRAMP structure in HT1080 cells. Time series corresponding to Figure 3C.
• Movie S9 - Rear polarity of WRAMP structure in WM239a cells. Time series corresponding to Figure 4A.
• Movie S10 - Rear polarity of WRAMP structure in A375 cells. Time series corresponding to Figure 4B.
• Movie S11 - Dynamic movement of WRAMP structure within WM239 and A375 cells as cells change direction. Time series corresponding to Figure 8F.