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Fig. 6. The knockdown of Dbs inhibits Schwann cell migration on fasciculated DRG axons after stimulation with NT3. Schwann cells were transfected with control or Dbs siRNA. Reaggregated Schwann cells on fasciculated DRG axons were incubated with NT3. DRG axons were stained for neurofilament (green), and the Schwann cells were stained with S100b (red).

Fig. 7. Dbs is not involved in the TrkC-mediated Rac1 activation in Schwann cells and Cos-7 cells. (A) Schwann cells were transfected with control or Dbs siRNA. (B) Cos-7 cells were transfected with Rac1 and TrkC ± FLAG-DbsD Cat. After stimulation with NT3, the Rac1 activity was measured with the pull-down assay by using GST-a Pak-CRIB in the lysates. Data were evaluated by using Student’s t test. *, P < 0.01.

Fig. 8. Schematic model for the signaling pathway coupling NT3 activation of TrkC to Schwann cell migration. Details are described in the main text. The specific GEF(s) responsible for Rac1 in the signaling pathway remain to be identified. It is possible that Cdc42 and Rac1 have effects on other cellular processes and may influence targets other than JNK upstream kinases.