Maximum-likelihood phylogenetic tree showing the relationship of Pelobacter sp. strain SFB93 to published Pelobacter strains and to other Deltaproteobacteria (Fig. S1); BRIG plot showing the similarity between the genomes of Pelobacter sp. SFB93 and P. acetylenicus DSM3246 and DSM3247, generated in this study (Fig. S2); phylogenetic tree of NifH amino acid sequences (Fig. S3); acetoin-grown washed cell suspensions of Pelobacter acetylenicus strain 3246 incubated with and without 2 mM NH4Cl under N2 with acetylene added to the gas phase (Fig. S4); similarity of genes in the acetylene fermentation pathway in genomes of Pelobacter sp. strain SFB93, Pelobacter acetylenicus DSM3246, and Pelobacter acetylenicus DSM3247 to genes in the GenBank database (Table S1); percent similarity between acetylene hydratase sequences from Pelobacter acetylenicus DSM3246 and DSM3247 and Pelobacter sp. SFB93 (Table S2); nitrogenase genes present in the genomes of Pelobacter sp. strain SFB93, Pelobacter acetylenicus DSM3246, and P. acetylenicus DSM3247 (Table S3); length of gap between nifHDKXB and nifEN genes in Pelobacter and Geobacter genomes presented in Fig. 2B (Table S4).
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