Tanga et al. 10.1073/pnas.0501634102.

Supporting Information

Files in this Data Supplement:

Supporting Table 1
Supporting Table 2
Supporting Figure 7

Table 1. Primer and TaqMan probe sequences for the mouse genes characterized in this experiment

Genes	Forward primers	Reverse primers	Probes*	GenBank accession no.
GAPDH	GGGAAGCTCACTGGCATGG	CTTCTTGATGTCATCATACTTGGCAG	TCCTACCCCCAATGTGTCCGTCGTG	XM_111622
CD11b (ITGAM)	GACCCTGTCCGCTCACGTATC	TCCACGCAGTCCGGTAAAATT	AACAACACACGCAGGCGCACCC	NM_008401
TLR4	AAACTTGCCTTCAAAACCTGGC	ACCTGAACTCATCAATGGTCACATC	CACGTCCATCGGTTGATCTTGGGAGAA	AF185285
CD14	CCAGTCAGCTAAACTCGCTCAATC	TCCAGCCTGTTGTAACTGAGATCC	AAGGGCTGCCAGCCAAGCTCAGC	BC057889
GFAP	GAGCGTGCAGAGATGATGGAG	TTCAGTTCAGCTGCCAGCG	ATCGAGAAGGTCCGCTTCCTGGAACAG	AF332062
IL-1β	GAAAGACGGCACACCCACC	AGACAAACCGCTTTTCCATCTTC	TGCAGCTGGAGAGTGTGGATCCCAA	M15131
TNF-α	GTTCTCTTCAAGGGACAAGGCTG	TCCTGGTATGAGATAGCAAATCGG	TACGTGCTCCTCACCCACACCGTCA	NM_013693
INF-γ	GATGCATTCATGAGTATTGCCAAGT	TTCCGGCAACAGCTGGTG	CAACCCACAGGTCCAGCGCCAAG	NM_008337

*The TaqMan probe has a reporter fluorescent dye, 6-carboxyfluorescein (FAM), at the 5' end, and a fluorescence dye quencher, 6-carboxytetramethylrhodamine (TAMRA), at the 3' end.

Table 2. Primer and TaqMan probe sequences for the rat genes characterized in this experiment

Genes	Forward primers	Reverse primers	Probes*	GenBank accession no.
GAPDH	CCCCCAATGTATCCGTTGTG	TAGCCCAGGATGCCCTTTAGT	TGCCGCCTGGAGAAACCTGCC	NM_01008
CD11b (ITGAM)	CTGCCTCAGGGATCCGTAAAG	CCTCTGCCTCAGGAATGACATC	CCCGGGACAATGCCGCGAA	U59801
TLR4	GATTGCTCAGACATGGCAGTTTC	CACTCGAGGTAGGTGTTTCTGCTAA	TCCTTGCTGAGGCAGCAGGTCGAAT	NM_0191178
CD14	GCAAACAGGTCGGCGTCTT	TGCGCAGCGCTAAAACTTG	TGATCTCGGCCCTCTGTCCGCA	AF087943
GFAP	TGGCCACCAGTAACATGCAA	CAGTTGGCGGCGATAGTCAT	CAGACGTTGCTTCCCGCAACGC	NM_017009
IL-6	GGGACTGATGTTGTTGACAGCC	CATATGTAATTAAGCCTCCGACTTGTG	TCACAGAGGATACCACCCACAACAGACCAG	NM_012589
IL-1b	GGAAGGCAGTGTCACTCATTGTG	GGTCCTCATCCTGGAAGCTCC	AAGCTGTGGCAGCTACCTATGTCTTGCCC	NM_031512
TNFa	CCCCGACTATGTGCTCCTCAC	AGGGCTCTTGATGGCGGA	CACACCGTCAGCCGATTTGCCACTT	AF329987

*The TaqMan probe has a reporter fluorescent dye, 6-carboxyfluorescein (FAM), at the 5' end, and a fluorescence dye quencher, 6-carboxytetramethylrhodamine (TAMRA), at the 3' end.

Supporting Figure 7

Fig. 7. Fluorescent-labeled confocal image of FITC-labeled oligonucleotide and DAPI-stained nuclei. This representative photomicrograph depicts the cellular uptake of FITC-labeled oligonucleotide. Twenty micrograms of FITC-labeled random oligonucleotide was injected intrathecally by means of lumbar puncture in three rats. The rats were killed at 4, 8, and 12 h after injection. Thirty-micrometer sections were colabeled with the specific nucleus marker DAPI, which is known to form fluorescent complexes with natural double-stranded DNA. The optimal cellular uptake of the FITC-labeled oligonucleotide (full arrow) was observed 8 h after injection in the proximity of nuclei (asterisk).