This PDF file includes:

• table S1. Target sequences recognized by established detection keys.
  
• table S2. Summary of a set of ARV factors targeted in this study.
  
• table S3. DNA sequences used in this study.
  
• fig. S1. Schematic of the plastic cartridge for RNA extraction.
  
• fig. S2. Comparison between the fluidic cartridge and a commercial column.
  
• fig. S3. The optical detection system in the PAD.
  
• fig. S4. Snapshots of a PAD application.
  
• fig. S5. The circuit diagram of the detection system.
  
• fig. S6. The effect of dUTP on the PAD assay.
  
• fig. S7. Portable PAD system.
  
• fig. S8. Lyophilized probes.
  
• fig. S9. Electrophoretic band-shift assay.
  
• fig. S10. Detection of ARV factors.
  
• fig. S11. Overall assay procedure for clinical samples.
  
• fig. S12. Universal and species-specific detection of HAI pathogens in clinical samples by the PAD system.
  
• fig. S13. Detection of ARV factors in clinical samples with PAD (top) and qPCR (bottom).
  
• References (29, 30)

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