A Novel Two-Component System, GluR-GluK, Involved in Glutamate Sensing and Uptake in Streptomyces coelicolor

Supplemental material

• Supplemental file 1 -

  Fig. S1, domain organization of GluR and GluK

  Fig. S2, amino acid alignment between GluR and its homologues

  Fig. S3, amino acid alignment between GluK and its homologues

  Fig. S4, verification of deletion of gluR-K in S. coelicolor

  Fig. S5, EMSAs of GluR binding to the intergenic region between gluABCD and gluR-K

  Fig. S6, analysis of gluR promoter activity

  Fig. S7, defining the GluR-binding sequence

  Fig. S8, analysis of gluR-K expression in the wild-type strain M145

  Fig. S9, prediction of transmembrane helices in GluK and AfsQ2

  Fig. S10, phenotypes of M145 and the ΔgluR-K mutant

  Fig. S11, biolayer interferometry analysis of binding to Trx tag

  Table S1, bacterial strains and plasmids

  Table S2, oligonucleotide sequences

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