Supplemental Materials
This article contains the following supporting material:
- Supplemental Materials
- Video S1 - Video 1. Time-lapse TIRF microscopy of a U2Os cell expressing RFP-EB1 (red) and Cenp-f^GFP (green). Arrowheads show Cenp-f^GFP localizing to the microtubule plus ends ahead of EB1 comets.
- Video S2 - Video 2. Mitochodria isolated from U2Os cells expressing mtBFP (blue) and depleted of CENP-F using RNAi were incubated with sfGFP-2592C (green) and added to dynamic microtubules (red) growing from GMPCPP-stabilized microtubule seeds (yellow).
- Video S3 - Video 3. A 1 μm glass bead coated with 2592C was attached to a growing microtubule using a laser trap. The trap was then shut down and bead and microtubule dynamics imaged using DIC optics.
- Video S4 - Video 4. A 1 μm glass bead coated with sfGFP-2592C (green) spontaneously attached to a dynamic HyLite647-labeled dynamic microtubule (red).
- Video S5 - Video 5. Dynamic microtubules assembled from HyLite647-labeled tubulin (red) growing from rhodamine-labeled, GMPCPP-stabilized microtubule seeds (blue) in the presence of 2 nM sfGFP-2592C (green). Arrows show tip-loading, non-motile sfGFP-2592C oligomers.