Host specificity analysis of SFB 16S rRNA gene sequences (Fig. S1); host specificity analysis of SFB flic1 at nucleotide and deduced amino acid levels (Fig. S2); staining of E. coli BL21 and Salmonella CVCC519 with DAPI and SFB FliC3 antibody (Fig. S3); expression of SFB FliC3 in mouse ileum mucosa and cecal content using FISH and IHC analysis (Fig. S4); relative location of SFB in gut epithelial tissue (Fig. S5); identification of genes related to flagellar assembly using LC/MC/MC in mouse gut samples (Fig. S6); peptides identified from purified mFliC3 bands (Fig. S7); phylogenetic analysis of TLR5 genes at the nucleotide and deduced amino acid levels (Fig. S8); rFliC3-enriched proteins related to the lysosome pathway (Fig. S9); degradation of mFliC3 and rFliC3 by rat ileum tissue proteins (Fig. S10); visualization of mutant variable regions of the deduced SFB FliC3 and FliC4 amino acid sequences (Fig. S11); summary PCR detection results for SFB genes (Table S1); diversity of mouse SFB flagellin genes (Table S2); diversity of rat SFB genes (Table S3); statistical analysis of NF-κB signaling data using multi-way analysis of variance (ANOVA) tests (Table S4); gene ontology analysis of the pull-down-enriched proteins (Table S5); number of actin-binding-related genes isolated during pull-down process (Table S6).
PDF, 2.3M