This PDF file includes:

• fig. S1. MEFs and hVSMCs coalign on planar surfaces.
  
• fig. S2. Apical and basal SFs align in distinct patterns in MEFs in response to curvature.
  
• fig. S3. Lengths of apical and basal SFs in hVSMCs on small and large cylinders.
  
• fig. S4. Phalloidin intensity of isolated hVSMCs on coverslips treated with CN03.
  
• fig. S5. F-actin organization is not recovered after CN03 washout.
  
• fig. S6. Basal SFs remain after inhibition of ROCK.
  
• fig. S7. Assembly of cylinder substrates.

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