This PDF file includes:

• table S1. Effects of MxB mutations at intermolecular interfaces 1, 3, and 4.
  
• fig. S1. Sequence alignment of Mx and dynamin proteins.
  
• fig. S2. MxB assembles into helical tubes with and without the MBP tag.
  
• fig. S3. GTPase activity of MBP-MxB at different protein and salt concentrations.
  
• fig. S4. MBP-MxB tubes in the presence of GTP without or with MgCl₂.
  
• fig. S5. CryoEM of the MBP-MxB assembly.
  
• fig. S6. Handedness of the MxB helical assembly map.
  
• fig. S7. Gold-standard Fourier shell correlation curve of the MBP-MxB density map.
  
• fig. S8. MDFF and real-space refinement of the MxB helical assembly model.
  
• fig. S9. Enlarged views of the intermolecular interfaces in the MxB assembly.
  
• fig. S10. Comparison of interfaces between cryoEM and crystal structures.
  
• fig. S11. Comparison of L1 and L2 contacts of MxB and dynamin 3.
  
• fig. S12. Interface 4 mutations relative to GTP-binding site.
  
• fig. S13. Comparison of MxB and MxA GTPase-BSE domains.

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Other Supplementary Material for this manuscript includes the following:

• movie S1 (.mp4 format). Molecular dynamics flexible fitting of MxB into the cryoEM map.
  
• movie S2 (.mp4 format). MxB assembles into a helical array.
  
• movie S3 (.mp4 format). Sα1c helix kinks from linear to tubular array.
  
• movie S4 (.mp4 format). Formation of Interface 3 during helical assembly.