Proteins LdhR and LdhA display conserved motifs of LTTRs and d-lactate dehydrogenases, respectively (Fig. S1), overexpression of genes ldhR and ldhA in B. multivorans ATCC 17616 accelerates the negative effect on cell viability (Fig. S2), buffering glucose-rich medium prevents extreme acidification and loss of cell viability and restores polysaccharide production by the wild-type B. multivorans (Fig. S3), and different sugars used as carbon sources support planktonic cellular aggregates formation (Fig. S4).
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Genes differentially expressed when the ∆ldhR transcriptome was compared with that of B. multivorans ATCC 17616, with both cell types grown in medium supplemented with 2% d-glucose (Table S1).
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