{"text_format":"2","workbook":"xlsx","n_sheets":2,"sheets":["Sheet1","Sheet2"],"not_rendered":{"charts":0,"images":0,"macros":0,"external_links":0,"defined_names":0,"comments":0,"pivot_tables":0},"unopened":[],"bounds":{"document_bytes":16777216,"sheet_cells":100000,"run_cost":"one","rows_first":2000,"rows_last":500,"rows_between":2000,"halvings":0,"cell_chars":16384,"cell_head_chars":4096},"cells_cut":0,"cells_raw":0,"read":"whole","date_system":"1900"}

{"sheet":"Sheet1","index":0,"n_rows":90,"n_cols":5,"rows_kept":90,"cut":false,"hidden":false,"merged":[[1,0,1,2],[33,0,33,2],[74,0,74,2]]}
["Additional file 1. All the primers used in this study, including 29 pairs of primers for precursor sequences,10 pairs of primers for quantitative real-time PCR of precursor sequences, and 6 pairs of primers for quantitative real-time PCR of target genes."]
["28 pairs of primers for precursor sequences "]
["miRNA name","forward primer(5’-3’)","reverse primer(5’-3’)"]
["miR156a","CAAGAGAAACGCAAAGAAACTGACA","AAAGAGATCAGCACCGGAATCTG"]
["miR157a","GTGTTGACAGAAGATAGAGAGCACAG","GGTGATGACAGAAGGCTAGAGAGC"]
["miR159a","GTAGAGCTCCTTAAAGTTCAAACAT","GTAGAGCTCCCTTCAATCCAAAG"]
["miR165a","GTTGAGGGGAATGTTGTCTGGATC","GTTGGGGGGGATGAAGCCT"]
["miR166a","AGGGGCTTTCTCTTTTGAGGGGACT","AGGGAGCAACAATTGGGGGGAATG"]
["miR167a","TGGTGCACCGGCATCTGATGAAGCT ","GGGTGCGACAGTCAACGGGTGAAAC",null," "]
["miR168a","CACCATCGGGCTCGGATT","CACCTCGAGGATCCGATTCAGT"]
["miR171a","ATGAGAGAGTCCCTTTGATATTGGC ","ACGAGAGAGTACTGAGATATTGGCG"]
["miR172a","TGCTGTGGCATCATCAAGAT","GCCGATGCAGCATCATCA"]
["miR319a","AGAGAGAGCTTCCTTGAGTCC","AGAGGGAGCTCCCTTCAGT"]
["miR319c","TAGATATAGAAGGAGATTCTTTCAG","TAGAAAAAGAAGGAGCTCCC"]
["miR390a","GTAGAGAAGAATCTGTAAAGCTCAG","GTAGTAAGAAGAGCCAATGAAACT"]
["miR394a","CTTACAGTCATCTTTGGCATTCTGT","CTAACAGATCTCTATTGGCAGTATACCC"]
["miR396a","CTCTGTATTCTTCCACAGCTTTCTT","GTCTGTATCTTCCCACAGCTTTATT"]
["miR396b","GGTCATACTTTTCCACAGCTTTCTTGAAC","TGTCATGTTTTCCCACAGCTTTCTTG"]
["miR403","TTGTCATTAGAAGAGTCGTATTACATGTT","AAAGACAGATTACGAGTTTGTGCGT"]
["miR408","AAGGTTAGATTGGTATTGCAATG","CGTTAGCAGTAGCAATAAAATTCC"]
["novel_1","GGAATGTTGTCTGGCTCGAGGAC","AGGGAATGAAGCCTGGTCCG"]
["novel_8","TGTGGGTTGTTGGGCAAGACCAT","GGTATGGGCGGTGTAGGCAAGAC"]
["novel_14","TTCCACAGCTTTCTTGAACTTCTTC","TTTCCCACAGCTTTCTCGAGC"]
["novel_23","TTATACAGAGAAATCACGGTCG ","CTTTATACAGAGAAACCCGGTC"]
["novel_25","AGGCACTTTCCCAGTCGTCT ","TCTGGCACTTCCAAGTCGTC"]
["novel_32","AGTGCATTGGACTATTG","CAAATGCATTGAAGTATTG"]
["novel_41","TGAAGCTGCCAGCATGAT","GGTGAAGCAACCACATGATC"]
["novel_45","TTTGATACGCACCTGAATCG","AATTTCGATACGAACCTAAATCG"]
["novel_46","TTTGGATCTCGGAAGTATAGTGG","GTTTGGATCTTGGAAAGTATAGAGG"]
["novel_68","GAGCTTCCTTCAGTCCACTCATG","AAAGGGAGCTCCCTTCAGTCC"]
["novel_84","AACGATGACGTTGTGTAATAGAT","TAGTTGTAACGATGACGTGGTG"]
[]
[]
["35  pairs of primers for quantitative real-time PCR of precursor sequences"]
["miRNA name","forward primer(5’-3’)","reverse primer(5’-3’)","RT primer(5'-3')"]
["miR156a-5p","AGCGCGCGTGACAGAAGAGAGT","ATCCAGTGCAGGGTCCGAGG","GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGTGCTC"]
["miR157a-5p","GCGCGCGCGTTGACAGAAGATAGA","ATCCAGTGCAGGGTCCGAGG","GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGTGCTC"]
["miR157d","GCGCGCGCGTGACAGAAGATAGA","ATCCAGTGCAGGGTCCGAGG","GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGTGCTC"]
["miR159a","GCGCGCGTTTGGATTGAAGGGA","ATCCAGTGCAGGGTCCGAGG","GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTAGAGC"]
["miR159b-3p","ACGCGCGCTTTGGATTGAAGGGA","ATCCAGTGCAGGGTCCGAGG","GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAAGAGC"]
["miR159c","GCGCGCGTTTGGATTGAAGGGA","ATCCAGTGCAGGGTCCGAGG","GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAGGAGC"]
["miR162a-3p","GCGCGCGTCGATAAACCTCTGC","ATCCAGTGCAGGGTCCGAGG","GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCTGGAT"]
["miR164a","CGCGTGGAGAAGCAGGGCA","ATCCAGTGCAGGGTCCGAGG","GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTGCACG"]
["miR166a-3p","CGCGTCGGACCAGGCTTCA","ATCCAGTGCAGGGTCCGAGG","GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGGGGAA"]
["miR167a-5p","GCGCGTGAAGCTGCCAGCAT","ATCCAGTGCAGGGTCCGAGG","GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTAGATC"]
["miR167d","ACGCGTGAAGCTGCCAGCATG","ATCCAGTGCAGGGTCCGAGG","GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCCAGAT"]
["miR168a-3p","GCGCCCGCCTTGCATCAA","ATCCAGTGCAGGGTCCGAGG","GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACATTCAG"]
["miR168a-5p","CGCGTCGCTTGGTGCAGGT","ATCCAGTGCAGGGTCCGAGG","GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTTCCCG"]
["miR319a","CGCGCGTTGGACTGAAGGGAG","ATCCAGTGCAGGGTCCGAGG","GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAGGGAG"]
["miR319c","CGCGCGTTGGACTGAAGGGAG","ATCCAGTGCAGGGTCCGAGG","GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAAGGAG"]
["miR390a-3p","GCGCGCGCTATCCATCCTGA","ATCCAGTGCAGGGTCCGAGG","GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTGAAAC"]
["miR390a-5p","AGCGCGAAGCTCAGGAGGGAT","ATCCAGTGCAGGGTCCGAGG","GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGGCGCT"]
["miR396a-3p","ACGCGCGCGGTTCAATAAAGCTGT","ATCCAGTGCAGGGTCCGAGG","GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCTTCCC"]
["miR396b-5p","GCGCGCGTTCCACAGCTTTCTT","ATCCAGTGCAGGGTCCGAGG","GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCAGTTC"]
["miR403-3p","AGCGCGCGTTAGATTCACGCACA","ATCCAGTGCAGGGTCCGAGG","GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCGAGTT"]
["novel_1","ACGCGTCGGACCAGGCTTCAT","ATCCAGTGCAGGGTCCGAGG","GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAGGGA"]
["novel_4","CGCGCGCGCGTCGACTGAAAATAAT","ATCCAGTGCAGGGTCCGAGG","GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGAATAG"]
["novel_6","CGCGTCGGACCAGGCTTCA","ATCCAGTGCAGGGTCCGAGG","GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGAGGAA"]
["novel_8","CGCGTCTTGCCTACACCGCC","ATCCAGTGCAGGGTCCGAGG","GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGGTATG"]
["novel_10","CGCGTTTCCAAGACCCCCCA","ATCCAGTGCAGGGTCCGAGG","GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACACGGTA"]
["novel_13","ACGCGTTTCCTACACCCCCCA","ATCCAGTGCAGGGTCCGAGG","GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGCGGAA"]
["novel_14","GCGCGCGTTCCACAGCTTTCTT","ATCCAGTGCAGGGTCCGAGG","GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAAGTTC"]
["novel_17","GCGCGCTGTGTTCTCAGGTCG","ATCCAGTGCAGGGTCCGAGG","GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCAGGGG"]
["novel_20","CGCGCGCGTTATGGAGAGGAACA","ATCCAGTGCAGGGTCCGAGG","GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCAACAT"]
["novel_27","CGCGCGTGGTATTGGTCCGG","ATCCAGTGCAGGGTCCGAGG","GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGCTGAG"]
["novel_32","GCGCGCGAGTGCATTGGACTATTGT","ATCCAGTGCAGGGTCCGAGG","GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCAAGGT"]
["novel_41","GCGCGTGAAGCTGCCAGCAT","ATCCAGTGCAGGGTCCGAGG","GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAAGATC"]
["novel_45","GCGCGCGTTTGATACGCACCTG","ATCCAGTGCAGGGTCCGAGG","GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCCGATT"]
["novel_68","ACGCGTTGGACTGAAGGGAGCT","ATCCAGTGCAGGGTCCGAGG","GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAAAGGG"]
["novel_72","CGCTTGCCGACACCACCCA","ATCCAGTGCAGGGTCCGAGG","GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTCGGAA"]
[]
["U6","GGAACGATACAGAGAAGATTAGCA","GTGCAGGGTCCGAGGT","GTGCAGGGTCCGAGGTTTTGGACCATTTCTCGAT"]
[]
[]
["6 pairs of primers for quantitative real-time PCR of target genes"]
["target ID","forward primer(5’-3’)","reverse primer(5’-3’)"]
["c50326_g1","TTATGGGAGGAATGAGATGGA","TTGTGGTTCACTGGCTTTCA"]
["c33977_g1","GCCAAACAAGAAGCAAGGTC","GTTGTGGGAGCGAATTTTGT"]
["c18176_g1","TTCAACCGAGGCTATGGAAC","ATACTGACCGAAAGCGGAAG"]
["c53763_g1","ATGAACTGCTCGCACACATC","TCTGGGGGACAGGAGTATGA"]
["c58954_g1","GAATGCCCAATTCTCTCCAA","GCCGTCAGCTTCCTATTCAC"]
["c44637_g1","TGCCCTTTACCCGAAATCTA","CATCCGTTCTCTTGCACCTT"]
["c47935_g1","CAACAGCTCCACGCACGTAT","CGTCAACGAACGGAGCCTAT"]
["c51834_g1","TTGGTGGTCTTGAGCAGAGG","GAGATCCAGGTGGTGGCTTC"]
["c82099_g1","TCAGCACCACTTGACTCCTCA","TTCCGGTGGCCTTCGATA"]
["c56307  g1","GGGATGGAGTCACCCACCAA","CCAGCATTCTCCCACCCAAG"]
["c58954  g1","CGCCGAGAGATGCTAGTCCT","CAGGCTTCATTCCAGGCATT"]
[]
[]
["ACTIN","CTTGCATCCCTCAGCACCTT","TCCTGTGGACAATGGATGGA"]

{"sheet":"Sheet2","index":1,"n_rows":0,"n_cols":0,"rows_kept":0,"cut":false,"hidden":false,"merged":[]}