Lysine acetylation stoichiometry and proteomics analyses reveal pathways regulated by sirtuin 1 in human cells

Supplemental Data

• Supplemental legends and figures
• Supplemental Table 1 -

  List of proteins and peptides identified acetylated in the cell lines HaCaT, CaLo and SiHa. The degree of acetylation occupancy was determined for the number of acetylation sites in the peptides. Coefficient of variation (CV%) and relative errors (RE%) for those acetylated peptides identified/quantified using the three sample preparation methods.
  

• Supplemental Table 2 -

  Pathway enrichment analyses performed with the online version of DAVID Bioinformatics software of the acetylated proteins identified in the three cell lines under study. Data are presented for each cell lines in different sheets.
  

• Supplemental Table 3 -

  List of proteins identified with at least two razor-unique peptides by MS/MS that were used for label-free quantification of the cell lines HaCaT, CaLo and SiHa. The ratios of LFQ intensities between cells is provided.
  

• Supplemental Table 4 -

  List of proteins and peptides that were found with less acetylation occupancy in SiHa cells in comparison with HaCaT and CaLo cells.
  

• Supplemental Table 5 -

  List of proteins and peptides that their acetylation stoichiometry was calculated (included those with no endogenous acetylation detected), for each of the six experimental conditions, the three cell lines (HaCaT, CaLo and SiHa) untreated and treated with the SIRT1 inhibitor EX-527. Data are presented for each condition in different sheets.
  

• Supplemental Table 6 -

  List of proteins and peptides that increased their acetylation occupancy in the three cell lines (HaCaT, CaLo and SiHa) after treatment with the SIRT1 inhibitor EX-527. The degree of acetylation for each condition was expressed in percentage of occupancy of at least one acetylation site.
  

• Supplemental Table 7 -

  List of peptides and proteins for each cell line that increased their acetylation occupancy by at least 5% after 24h treatment with the SIRT1 inhibitor EX-527. The LFQ intensities for each protein as well as the ratios of LFQ intensities between cells is provided. Data are presented for each cell lines in different sheets.
  

• Supplemental Table 8 -

  List of proteins identified with at least two razor-unique peptides by MS/MS that were used for label-free quantification of the cell lines HaCaT, CaLo and SiHa, untreated and treated with the SIRT1 inhibitor EX-527. The ratio of LFQ intensities between conditions is provided.