Protective Vaccine Efficacy of the Complete Form of PPE39 Protein from Mycobacterium tuberculosis Beijing/K Strain in Mice

Supplemental material

• Supplemental file 1 -

  Fig. S1. Diagram of genetic variation PPE39 among M. tuberculosis Beijing/K, M. tuberculosis H37Rv, and M. bovis BCG Pasteur 1173.

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• Supplemental file 2 -

  Fig. S2. Protective efficacy of immunization with MTBK_24820 in mice against the M. tuberculosis Erdman strain.

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• Supplemental file 3 -

  Fig. S3. (A) Recombinant MTBK_24820 was overexpressed and purified in E. coli using histidine affinity chromatography followed by an FPLC purification method. (B) Synthetic peptides overlapped by six amino acids including the N terminus of MTBK_24820 were designed and dissolved in PBS.

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• Supplemental file 4 -

  Fig. S4. Gating strategy using Flowjo program.

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