Supplemental Materials
This article contains the following supporting material:
- Supplemental Materials
- Video 1 - Centrosome separation in otherwise wild�type one-cell stage C. elegans embryos expressing GFP::TAC-1, imaged using 3D time-lapse DIC and fluorescence microscopy (GFP), as reported in (De Simone et al., 2016). The single focal plane DIC channel and a projection of all focal planes in the GFP channel are shown together; centrosomes are highlighted (small blue and red foci), as are the male (red disc) and female (blue disc) pronuclei. Time is indicated in seconds, with 0 s defined as the earliest time-point in which the two separate centrosomes could be detected in the whole synchronized control dataset in (De Simone et al., 2016); scale bar = 10 μm. Note that the small initial apparent size of the female pronucleus compared with the male pronucleus is due to the former being initially located outside of the imaged volume. Similarly, a centrosome cannot be detected from t = 341 to 365 s because it moved out of the imaged volume.
- Video 2 - Computer simulations of centrosome separation in goa-1/gpa-16(RNAi) embryos, as such (top), as well as with the male pronucleus and associated centrosomes located centrally and a lack of microtubule-centrosome steric interaction (bottom). Nuclear dynein (blue dots), male pronucleus (blue sphere), centrosomes (green dots), microtubules (white lines) and cortex (light grey ellipse in transparence) are depicted. For visual clarity, unbound motors are hidden and only 1/4 microtubules is shown. See text, as well as Materials and Methods, for more information.