Supplemental Materials
This article contains the following supporting material:
- Supplemental Materials
- Video 1 - Confocal time series of Syk-KO RBL cells expressing SykmNG-WT (green) primed with AF647-IgE (magenta) after crosslinking with 0.1 μg/mL DNP-BSA. Scale bar 5 μm, video played at 60x speed. Video corresponds to the image sequence in Figure 1A.
- Video 2 - Large aggregates of SykmNG that form after addition of DNP-BSA are quickly (within seconds) dispersed into individual SykmNG molecules after addition of 100 μM monovalent DNP-lysine. Images acquired in TIRF and displayed at 2x real time, played at 20 frames/sec. Scale bar 1 μm. Video corresponds to the image sequence in Figure 1E.
- Video 3 - Example image series of single molecule SykmNG-WT and corresponding trajectories after DNP-BSA addition. Image (greyscale) overlaid with identified single particle trajectories colored by track length. Video played in real time at 10 frames/sec. Scale displayed on axes.
- Video 4 - Overlay of SykmNG trajectories (green lines) with AF647-IgE (magenta) aggregates in cells stimulated with 0.1 μg/mL DNP-BSA for 4 min. Trajectories show localizations from the last 20 frames. Video played in 2x real time at 20 frames/sec. Scale bar 1 μm. Video corresponds to image in Figure 3F (left).