The Type IX Secretion System Is Required for Virulence of the Fish Pathogen Flavobacterium columnare

Supplemental material

• Supplemental file 1 -

  Photomicrographs of wild-type and mutant F. columnare cells on glass at time 0 from movies used to generate “rainbow traces” in Fig. 4 (Fig. S1); secretion of the foreign protein mCherry fused to the C-terminal domain of F. johnsoniae ChiA by F. columnare T9SS (Fig. S2); survival of zebrafish exposed to different dilutions of wild-type F. columnare C#2, T9SS mutants, and complemented strains (Fig. S3); growth curves of wild-type, mutant, and complemented F. columnare strains (Fig. S4); survival of rainbow trout and catfish exposed to different dilutions of wild-type F. columnare, T9SS mutants, and complemented strains (Fig. S5); F. columnare type IX secretion system protein-encoding genes (Table S1); legends to supplemental movies.

  PDF, 2.0M

• Supplemental file 2 -

  F. columnare C#2 and IAS-4 genes encoding proteins with C-terminal domains predicted to target them for secretion by the type IX secretion system (Table S2).

  XLSX, 16K

• Supplemental file 3 -

  Movement of cells of wild-type F. columnare strain IA-S-4, the ΔgldN mutant, and the ΔgldN mutant complemented with wild-type gldN on pLN5 on glass coverslips (Movie S1).

  MOV, 14M

• Supplemental file 4 -

  Movement of cells of wild-type F. columnare strain C#2, the ΔgldN mutant, and the ΔgldN mutant complemented with wild-type gldN on pLN8 on glass coverslips (Movie S2).

  MOV, 14M

• Supplemental file 5 -

  Movement of cells of wild-type F. columnare strain C#2 and the ΔporV mutant on glass coverslips (Movie S3).

  MOV, 9.6M