Supplemental Materials
This article contains the following supporting material:
- Supplemental Materials
- Movie S1 - Supplemental Movie S1: Z-�plane imaging of Drosophila embryo in microfluidic device Movie showing plane--by--plane a fixed Drosophila embryo in cycle 14 expressing H2Av::mCherry (red) and Gap43::mVenus (green), with the microfluidic chamber visible in grayscale.
- Movie S2 - Supplemental Movie S2: Stereographic projection of embryo anterior pole Movie through the slices of the anterior pole after the stereographic projection of a fixed embryo expressing Gap43::mVenus in late slow phase. First frame corresponds to near apical surface. Last few frames correspond to basal surface. The anterior--most cells are slightly shorter than the other cells (hence dark region at end of movie in the center).
- Movie S3 - Supplemental Movie S3: Close up of segmented cells undergoing cellular rearrangements along apical-�basal axis
- Movie S4 - Supplemental Movie S4: Laser ablation on cell apical surface Laser ablation of Drosophila embryo expressing Gap43::mVenus toward end of slow phase. Ablation in trunk region. Refer to 1 for detailed methods.
- Movie S5 - Supplemental Movie S5: fat2-�RNAi embryo expressing Gap43-�mCherry during cycle 14 UAS>fat2--RNAi, trafficjam>Gal4, Gap43::mCherry embryo through cycle 14. Orientated with anterior to left and ventral side at the bottom. Gastrulation appears to occur normally.
- Movie S6 - Supplemental Movie S6: 3D vertex model -� apical junctions near the head 3D vertex model evolution of the apical junctions near the head � modeled as an ellipsoid geometry --during the cell depth relaxation phase: (left column) for a cell depth h = 10 μm, corresponding to an early phase during cellularization, and (right column) for a cell depth h = 20 μm;; (upper rows) color coding represents neighbor number of each apical faces: green for hexagons, blue for pentagons, and yellow for heptagons. The apical T1 transitions that appear for h = 20 μm units relax at long time scale. (lower rows) 3D tissue structure: apical (blue) and basal (red) networks.
- Movie S7 - Supplemental Movie S7: 3D vertex model � apical junctions near the trunk As Supplementary Movie 6, but with a cylindrical geometry.