Supplemental Materials
This article contains the following supporting material:
- Supplemental Materials
- Movie S1 - Movie S1, Related to Figure 4. Anaphase onset in a HeLa cell transiently expressing GFP-INCENP and MKlp2-mCherry. Single optical sections were collected every 3 seconds. Playback is at 10 frames per second (fps).
- Movie S2 - Movie S2, Related to Figure 4. Anaphase onset in HeLa cells transiently expressing GFP-INCENP. Cells were treated with a control (left) or MKlp2-targeting (right) siRNA. Single optical sections were collected every 3 seconds. Playback is at 10 fps.
- Movie S3 - Movie S3, Related to Figure 4. Anaphase onset in a HeLa cell transiently expressing GFP-PRC1 and MKlp2-mCherry. Single optical sections were collected every 5 seconds. Playback is at 10 fps.
- Movie S4 - Movie S4, Related to Figure 4. Anaphase onset in a HeLa cell transiently expressing GFP-KIF4A and MKlp2-mCherry. Single optical sections were collected every 5 seconds. Playback is at 10 fps.
- Movie S5 - Movie S5, Related to Figure 4. Anaphase onset in a HeLa cell transiently expressing INCENP-PA-GFP. The first frame represents the time point immediately after photoactivation. The final frame represents activation of total cellular INCENP. A maximum Z-projection was generated from 3 optical sections (1 μm sections) of INCENP-PA-GFP, and a single optical section was collected in DIC to visualize chromosomes. Images were collected every 30 seconds. Playback is at 5 fps.
- Movie S6 - Movie S6, Related to Figure 5. C phase progression in a HeLa cell plated on 100 μg/ml Fibronectin transiently expressing GFP-INCENP and MKlp2-mCherry. Single optical sections of the midzone were collected every 5 seconds. Playback is at 10 fps.
- Movie S7 - Movie S7, Related to Figure 6. C phase progression in a HeLa cell plated on 100 μg/ml Fibronectin transiently expressing GFP-INCENP and MKlp2-mCherry. Single optical sections in the plane of the cortex were collected every 5 seconds. Playback is at 10 fps.
- Movie S8 - Movie S8, Related to Figure 7.Furrow ingression in HeLa cells transiently expressing mCherry-Utrophin to mark the cleavage furrow. Cells were treated with a control (left) or MKlp2-targeting (right) siRNA. Single optical sections were collected every 30 seconds to prevent photobleaching. Playback is at 10 fps.
- Movie S9 - Movie S9, Related to Figure 7.Furrow ingression in HeLa cells transiently over-expressing GFP-INCENP. Cells were treated with a control (left) or MKlp2-targeting (right) siRNA. Single optical sections were collected every 5 seconds. Playback is at 10 fps.
- Movie S10 - Movie S10, Related to Figure 7. Furrow ingression in HeLa cells transiently over-expressing GFP-INCENP CR. Cells were treated with a control (left) or MKlp2-targeting (right) siRNA. Single optical sections were collected every 5 seconds. Playback is at 10 fps.