Conjugation-Mediated Horizontal Gene Transfer of Clostridium perfringens Plasmids in the Chicken Gastrointestinal Tract Results in the Formation of New Virulent Strains

Supplemental material

• Supplemental file 1 -

  Conjugation frequencies from in vitro-constructed transconjugants (Table S1); alignment of pJIR3535 and pNetB-Erm^R (Fig. S1); PFGE confirmation of in vitro-derived BER-NE33 (Fig. S2) and PBD1 (Fig. S3) transconjugants; Simply blue-stained SDS-PAGE protein gel of TCA-precipitated culture supernatants from actively growing cultures of C. perfringens strains EHE-NE18, WER-NE36, BER-NE33, and PBD1 and four transconjugants (Fig. S4); PFGE confirmation of in vivo-derived transconjugants isolated from co-challenge with BER-NE33 and WER-NE36 (Fig. S5).

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