This PDF file includes:

• method S1. Calculation of the focal spot size of TRUE and conventional focusing.
  
• method S2. Viral injection surgery.
  
• method S3. Electrophysiological recordings.
  
• method S4. Daily alignment procedure.
  
• fig. S1. Setup.
  
• fig. S2. Electrical signal flow diagram.
  
• fig. S3. Ultrasound pulse-echo image of the tip of the glass pipette electrode.
  
• fig. S4. Electrophysiological photocurrent traces from neurons in 500- and 300-μm-thick acute brain slices.
  
• fig. S5. Electrophysiological photocurrent and membrane voltage traces comparing ultrasound on and off conditions.

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