Supplemental Materials
This article contains the following supporting material:
- Supplemental Materials
- Movie S1 - Movie S1. The P4 blastomere does not complete cellular abscission. Related to Figure 1A. Timelapse movie of a control embryo expressing NMY-2::GFP (green), a membrane marker (TagRFP::PH, magenta) and a germ granule marker (PGL-1::RFP, magenta). Z stacks (0.5 μm each) were acquired every 35 s and a maximum intensity projection of 3 consecutive stacks is shown for each frame. In somatic cells (lacking PGL-1::RFP signal), the NMY-2-positive midbody ring that forms after cytokinesis is released and degraded within minutes but remains stably present at the interstitial membrane separating the two PGCs (PGL-1::RFP-positive cells). Playback rate is 140x real time (four frames/second). Anterior is to the left. Scale bar, 10 μm.
- Movie S2 - Movie S2. P4 division into Z2 and Z3 in control or ANI-2-depleted embryos. Related to Figure 3B. Time-lapse movies of control (left) and ani-2(RNAi) (right) embryos expressing a membrane marker (mNG::PH, green) and a nucleus marker (mCherry::H2B, magenta). Z stacks (0.75 μm each) were acquired every 180 s and a maximum intensity projection of 2 consecutive stacks is shown for each frame. The membrane between Z2 and Z3 is stable in control embryos but collapses during rotation in ANI-2-depleted embryos. Playback rate is 720x real time (four frames/second). Anterior is to the left. Scale bar, 10 μm.