Sld5 Ensures Centrosomal Resistance to Congression Forces by Preserving Centriolar Satellites

Supplemental material

• Supplemental file 1 -

  Movie S1 (Control GL2 siRNA-transfected cells displayed normal spindle pole organization)

  AVI, 67K

• Supplemental file 2 -

  Movie S2 (Sld5-depleted cells appeared normal until chromosome condensation in prophase, followed by abnormal prometaphase)

  AVI, 45K

• Supplemental file 3 -

  Movie S3 (In control GL2 siRNA-transfected cells, chromosomes congressed to form metaphase plate)

  AVI, 104K

• Supplemental file 4 -

  Movie S4 (In Sld5-depleted cells, unaligned chromosomes did not congress to equatorial plate)

  AVI, 82K

• Supplemental file 5 -

  Movie S5 (Live-cell imaging of GL2 or SLD5 siRNA-transfected cells stably coexpressing H2B mCherry and mEGFP–α-tubulin)

  AVI, 118K

• Supplemental file 6 -

  Movie S6 (Live-cell imaging of GL2 or SLD5 siRNA-transfected cells stably coexpressing H2B mCherry and mEGFP–α-tubulin)

  AVI, 18M