Promoter region upstream of ectA present on the ect-lacZ fusion plasmid pGJK4 (Fig. S1); site-directed mutagenesis of an inverted repeat located upstream of the ectA promoter (Fig. S2); transcriptional activity of the ect promoter variant Mut 15 in response to increasing osmolarity (Fig. S3); Western blot analysis of ect-lacZ reporter fusions of the wild-type ect promoter and two of its variants (Fig. S4); comparison of ectoine and hydroxyectoine production and extracellular accumulation (Fig. S5); osmolarity of the medium used for cultivation (Fig. S6); effects of plasmid-based ectoine/hydroxyectoine production on the growth of the E. coli strain SK51 (Fig. S7).
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