The Swinholide Biosynthesis Gene Cluster from a Terrestrial Cyanobacterium, Nostoc sp. Strain UHCC 0450

Supplemental material

• Supplemental file 1 -

  Swinholide A through K and isoswinholide A and B structures and exact monoisotopic masses (Fig. S1A); fragmentation analysis of MS^E spectra of commercial swinholide A and the compound from Nostoc sp. strain UHCC 0450 (Fig. S1B); NMR analysis of the methanol extract of Nostoc sp. UHCC 0450 (Fig. S2); chromatograms and product ion spectra of sodiated swinholide molecules produced by Nostoc sp. UHCC 0450 (Fig. S3); maximum-likelihood tree of ketosynthase domains 6 to 17 and 19 of the six studied gene clusters (Fig. S4); ¹H and ¹³C NMR data for swinholide A from Nostoc sp. UHCC 0450 in D₆-DMSO and CD₃OD, swinholide A in CD₃OD and CDCl₃, swinholide F in CDCl₃, isoswinholide A in CDCl₃, and isoswinholide B in CD₃OD (Table S1); retention times, ion masses, and relative intensities of sodiated molecules of swinholide variants 1 to 6 produced by Nostoc sp. UHCC 0450 (Table S2); most similar hits in BLASTp searches of the ORFs in the swinholide cluster and surrounding ORFs in Nostoc sp. UHCC 0450 (Table S3A); most similar hits in BLASTp searches of the ORFs in the scytophycin cluster and surrounding ORFs in Anabaena sp. UHCC 0451 (Table S3B); distance matrices of the six related macrolide biosynthesis gene cluster core genes (Table S4); HGT events detected in HGTector for the PKS modules (Table S5); information used to construct the phylogenetic acyltransferase tree (Table S6).

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