Fig. S1 (Genetic variation in nucleotide position 163 of the VP1 region of WPV1 by year of emergence and by genetic cluster), S2 (Sensitivity of WPV1 assay), S3 (Sensitivity of PV2 assay), and S4 (Flow chart of ITD 5.0 algorithm for testing virus isolates with rRT-PCR assay) and Tables S1 (Statistical probability of positivity in each rRT-PCR assay by target, template, and concentration of virus), S2 (Statistical probability of positivity for each rRT-PCR assay by target, template, and concentration of RNA transcripts), S3 (Sequences of primers and components used for RNA transcript library), S4 (Primers and primer sequences used for whole-genome amplification of polioviruses serotypes 1, 2, and 3), and S5 (Reference viruses by sequence identification number, strain, serotype, and accession number in GenBank)
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