LOCUS punc-17::ACR-1:: 8258 bp DNA circular SYN 17-OCT-2017 DEFINITION Ligation of pFUGW-hGtACR1-EYFP** into RM#348p* ACCESSION punc-17::ACR-1:: KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 8258) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by SciEd Central, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: RM#348p* Fragment ends: HincII and NheI Fragment size: 6631 Insert molecule: pFUGW-hGtACR1-EYFP** Fragment ends: XbaI and blunt Fragment size: 1627 FEATURES Location/Qualifiers CDS 51..3360 /gene="punc-17 (promoter)" /SECDrawAs="Gene" CDS 3366..4976 /gene="ACR1::YFP" /SECDrawAs="Gene" CDS 4260..4976 /gene="eYFP" /SECDrawAs="Gene" ORIGIN 1 atgaccatga ttacgccaag cttgcatgct cccggccgcc atggccgcgg gattacacca 61 atcatttctc ccctccgaaa tgtgtagtcg tcatgacaaa gtggtgacac tggattacgg 121 taggctccgc ccacttcttg gaaaagtgac gtgggctcgg tgagccctaa agcttttggt 181 tttcacaatt ttctggtttt tgggattttt gcgggaattt tgagaggaga aagttgggag 241 agaagatatt ggaatggtcc ccggggagct gtacatttat acatctagac tgaagctggg 301 gtattgcaaa aagagaaaag cctatcactt tctttccatc ctgcatactt gccaaaaagt 361 tttggctgag cttattttta aaattggaat tttttggaat tttcagaaaa gaaaaagttt 421 tttctttttt tttaatttaa aaaatttttg ctatttattg catggtacta cagaaaaact 481 tgaatgaaat aaaaaatttg gaaaattcaa gaaaactttt tttttcatag tttatttatt 541 cgagttttaa tgtggctttt attttgttcg gtttttgttt taaaaagatt ttatacaaaa 601 tttcaagaat ttacaatttt tccgttgtaa aaaatccaaa aaaaaatcca aaaaaatagt 661 tcagaccttt ttttaaattt taaatttaaa aattcaatac caaaaaatta aaaaaaatta 721 gtttttccga gaataaagga aaaatgttga aaaaatatta tatataaaat ttccagaaaa 781 tgtgaaaact ccaaaaaact gcagactttt ccccaaacta gctatttttt gaattcatta 841 cctttaaagg tggagtaccg aaatttaaga ctttgctttt ttagacccaa aatggtccaa 901 aactaccaaa tttcgtaatg agacgttctg aaaatttttc aaaaaaatgt tatgggagct 961 caaagttttt gaaaaaatgg tatattttta gctaatttta accacttctt ggcgtcgcag 1021 cggttggaac tcaattttaa ttaaattatc atccttcaat gtccttcaat ctttgcaatt 1081 tcaatcgacc atacgcgttc agagcatttt tttttttcgt ttttcctccc gtccagcaca 1141 tatttaaata ttctctctcc ccctcccctg gttcctaaaa acttgggaaa ggggaatttc 1201 gaaggaggtg gaagcttctt cttctccccg agcacacaca cacatcctct cttcaaaatc 1261 tttaagaaat agtgcgtgtg aatgactaga cgctgaagct aacttggttt ccgcgaacaa 1321 tcccaatcag tgcctgtgcc gaggcttcgg agaagaagag attgacgtcg tctttttctt 1381 tttttgtgaa acagaataaa aaagaatacg aaatggactt tttcacacat tttcgttact 1441 tttccagaaa aaaacgaagc tctagccatg gaagtttgaa ttgaaaacct aaaccccgat 1501 gtgtattcat ttgagcttaa gcctaagcct aattctacgc ctaagcataa gcctaagcct 1561 atgttcaagc ctaagccaaa ttgtagaacg ttaccaaaag atcaaaccta tttttcctac 1621 agaaaaggtc taatgataac tgttaagcga gagacaagga gagatatata tcaaaaacta 1681 gcccaaactg tgattaacat ctattaactg acctcaaaaa aaaaaatagt agagattaaa 1741 tactgctcaa aaagtttctt ggaaaagtaa attgttaggt ttctggggcc tgtaaaactg 1801 gaccgagttt tgaagaattt ttttaacttt tcaaagtttg gcgtcactga aagctttata 1861 ttgtacatgt tccaccaatt ttcttttgta tccgtttttt ctgtctcaaa acggagctcg 1921 agcccatgac gtgtggatac cgtgagaatc cgcccaattc cttcacatcg atctcatatt 1981 tctttcccag tttatattga attcccgttg aattgcggct ttgcagggga gaagatgtgc 2041 gtctttatat ttttttttct atccttaccc gccgtgaatc cggctcgaat tgagccaatt 2101 tttatgaata gtagagctaa ttgacttcgt ctccctagaa ttgatgaggt aaagctcgtg 2161 gggagatatg gagatttttc gatttttttt ccagtaattt tcagttcaaa atttgagctt 2221 ttgagcagga gttttgagat ttgtgccaaa atttcctatt attttttcaa atatttttgc 2281 ttttgtaaaa aaggtgtagt agaagttttg tagtgccact attttttttt tgaaaattat 2341 gttttggcgg aaagttcttt aaaaaagtta atcaaaattt ttggcgcttt agtcaaatta 2401 attttctggc aaaaatatta gtaattttgg cgtgagttca attttcccat tcttaaaatt 2461 tcacacaatt aagaatttta agatttgggt ttttttttca acaaaaaatc aaatatttac 2521 atgaaaattc ggcgggaaat aaaaaaaaat attccgattc aatgtgtgaa taactttttg 2581 cggggaattc aaatttgcat tatttaaact ttttttaagc tcgaaaaatt gaaattctta 2641 aataaataaa taaaattttc ctcgaatttt gaatgtttcg attttttagc gttatttttt 2701 caaaatttcc actaaaactc tagttctaaa gttctaaaac tttaaaaact tcctaatgtt 2761 gtggtcttgt gtttttttgg cagcgatttc caaatttctg aaattccaac aaaaaaacca 2821 ctacgtcagt ttgcatatcc aacccccccc cccccccccc cccctctgag ccaatcaatc 2881 atgtgcattt tgaattggcg ccaaggcaat cttcgtaaaa aataacaata gagtcatccc 2941 tcagaacaga aaagaaaccc gcccgggggc tcaaatacac atgtcatttt tcttttttcg 3001 cctgattttt agcctaaatt agtgtttagc aattttttaa ggtaactcgg tggagtcatt 3061 tgaacttttt aaactaaaat ttcagaaaaa attctttttt tttgtcaaaa aataattttc 3121 ccgccggaat ttttcaaaaa aataattttt attttatttt tcaagttcct tgtttcctct 3181 ttattttttt attttgcccc caaaagtttt acactaaaat atttaatttt caggtaattc 3241 atcgaaagtc tttctatttt ccgcatctct tgttcaaggg attggccaaa ggacccaaag 3301 gtatgtttcg aatgatacta acataacata gaacattttc aggaggaccc ttggctagag 3361 gatccatgag cagcatcacc tgtgatcccg ccatctacgg cgaatggagc agggagaacc 3421 agttctgcgt ggagaagagc ctgatcaccc tggacggcat caagtacgtc cagctggtga 3481 tggccgtcgt gagcgcctgt caggtgttct tcatggtgac cagagccccc aaggtgccct 3541 gggaagccat ctacctgccc accaccgaga tgatcaccta ttccctggcc ttcaccggaa 3601 acggctacat cagagtggcc aatggcaagt acctgccctg ggccagaatg gccagctggc 3661 tgtgcacctg ccctatcatg ctgggcctgg tgagcaatat ggccctcgtg aagtacaaga 3721 gcatccccct caaccctatg atgatcgccg cctccagcat ctgcaccgtg ttcggcatca 3781 ccgcctccgt ggtgctagac ccgctgcacg tgtggctgta ctgtttcatc agcagcatct 3841 tcttcatctt cgagatggtg gtggccttcg ccattttcgc cattaccatc cacgatttcc 3901 agaccatcgg ctcccccatg tccctgaagg tggtggagag gctgaagctg atgaggatcg 3961 tgttctacgt gagctggatg gcctacccta tcctgtggag cttctccagc accggcgcct 4021 gcatcatgag cgagaacacc agcagcgtgc tgtacctgct gggcgacgct ctgtgcaaga 4081 acacctacgg catcctgctg tgggctacaa cctggggcct gctgaacggc aagtgggaca 4141 gggattacgt gaagggcagg aacgtggacg gcaccctgat gcctgagtac gagcaggacc 4201 tggagaaggg caacaccgag aggtacgagg acgccagagc cggcgagacc gcggccgccg 4261 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg 4321 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca 4381 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg 4441 tgaccacctt cggctacggc ctgcagtgct tcgcccgcta ccccgaccac atgaagcagc 4501 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca 4561 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga 4621 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc 4681 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca 4741 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc 4801 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc 4861 tgagctacca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc 4921 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaagaat 4981 tgacggtacc atggtattga tatctgagct ccgcatcggc cgctgtcatc agatcgccat 5041 ctcgcgcccg tgcctctgac ttctaagtcc aattactctt caacatccct acatgctctt 5101 tctccctgtg ctcccacccc ctatttttgt tattatcaaa aaaacttctt cttaatttct 5161 ttgtttttta gcttctttta agtcacctct aacaatgaaa ttgtgtagat tcaaaaatag 5221 aattaattcg taataaaaag tcgaaaaaaa ttgtgctccc tccccccatt aataataatt 5281 ctatcccaaa atctacacaa tgttctgtgt acacttctta tgtttttttt acttctgata 5341 aatttttttt gaaacatcat agaaaaaacc gcacacaaaa taccttatca tatgttacgt 5401 ttcagtttat gaccgcaatt tttatttctt cgcacgtctg ggcctctcat gacgtcaaat 5461 catgctcatc gtgaaaaagt tttggagtat ttttggaatt tttcaatcaa gtgaaagttt 5521 atgaaattaa ttttcctgct tttgcttttt gggggtttcc cctattgttt gtcaagagtt 5581 tcgaggacgg cgtttttctt gctaaaatca caagtattga tgagcacgat gcaagaaaga 5641 tcggaagaag gtttgggttt gaggctcagt ggaaggtgag tagaagttga taatttgaaa 5701 gtggagtagt gtctatgggg tttttgcctt aaatgacaga atacattccc aatataccaa 5761 acataactgt ttcctactag tcggccgtac gggccctttc gtctcgcgcg tttcggtgat 5821 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg 5881 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc 5941 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa 6001 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc ggccttaagg gcctcgtgat 6061 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac 6121 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat 6181 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag 6241 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc 6301 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc 6361 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc 6421 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc 6481 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt 6541 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt 6601 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat 6661 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct 6721 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat 6781 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc 6841 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg 6901 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc 6961 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta 7021 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc 7081 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga 7141 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat 7201 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat 7261 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa 7321 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa 7381 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt 7441 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt 7501 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata 7561 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt 7621 ggagcgaacg acctacaccg aactgagata cctacagcgt gagcattgag aaagcgccac 7681 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga 7741 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg 7801 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa 7861 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat 7921 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc 7981 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga 8041 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg 8101 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta 8161 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg 8221 aattgtgagc ggataacaat ttcacacagg aaacagct //