Supplemental text: NMR data; SBOL presentations of plasmids (Fig. S1); expression of the putative chrysogine gene cluster in strain DS68530 and the ΔPc21g12640 mutant (Fig. S2); chromatograms of culture broths from DS68530 and gene deletion strains after 96 h of growth in SMP medium (Fig. S3); mass spectra of uncharacterized chrysogine-related compounds (Fig. S4); 1H NMR spectra of compounds 3, 8, 13, 1, and 4 (Fig. S5); HSQC spectra of compounds 3, 8, and 13 (Fig. S6); HMBC spectrum of compound 4 and impurity compound 2 (Fig. S7); chemical shifts of compounds 3, 8, and 13 (Table S1) and of compounds 1, 4, and 2 (Table S2) in 1:1 DMSO/CDCl3.
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