FungiOphiostomatalesOphiostomataceaeChangRunleiDuongTuan A.TaerumStephen J.WingfieldMichael J.ZhouXudongde BeerZ. WilhelmOphiostomatoid fungi associated with conifer-infesting beetles and their phoretic mites in Yunnan, ChinaMycoKeys21122017201728196410.3897/mycokeys.28.21758 Ophiostoma brevipilosi 823694 R.Chang & Z.W.de Beersp. nov.Fig. 8Etymology.

The epithet brevipilosi refers to the bark beetle vector Tomicus brevipilosus from which all eight isolates of this taxon were obtained.

Description.

Sexual state not observed. Pesotum-like macronematal asexual state predominant. Synnemata simple, dark brown at the base, (179.5–) 227–468 (–667) μm long including conidiogenous apparatus, (22–) 32.5–58 (–69) μm wide at base; cells (13–) 16–26 (–32.5) μm long, conidia hyaline, 1-celled, smooth, oblong, (3–) 3–4.5 (–5.5) × (1.5–) 1.5–2.5 (–3) μm. Hyalorhinocladiella-like asexual state: conidiophores (14.5–) 33–115 (–145) μm long; cells arising directly from the hyphae, (12–) 15–38 (–47) × (1.1–) 1.5–2 (–2.5) μm; conidia hyaline, smooth, obovoid, (2.5–) 3–5.5 (–8) × (1.5–) 2–2.5 (–3) μm.

Morphological characters of asexual structures of Ophiostoma brevipilosi sp. nov. a fourteen days old culture on OA b–d Hyalorhinocladiella-like asexual state and condia e Pesotum-like macronematal asexual state f cells of Pesotum-like macronematal asexual state g conidia. Scale bars: a–g = 10 μm.

Culture characteristics.

Colonies hyaline at the beginning, then becoming white to dark. Mycelium superficial on the 3% OA. Colony margin smooth. Colonies on 2% MEA flat, reaching 67 mm diam in 11 d at 25 °C. No growth observed at 5 and above 30 °C. Optimal temperature for growth 20 and 25 °C.

Type material.

CHINA, Yunnan Province, Puer City, from Tomicus brevipilosus on Pinus kesiya bark, 27 Jun. 2010, S.J.Taerum, herbarium specimen of dried culture, PREM 61537 (holotype), CMW41873 = CBS139660 (ex-holotype culture).

Additional specimens examined.

CHINA, Yunnan Province, Puer City, from Tomicus brevipilosus on Pinus kesiya bark, 27 Jun. 2010, S.J.Taerum, PREM 61538, CMW41624 = CBS139661; CHINA, Yunnan Province, Puer City, from Tomicus brevipilosus on Pinus kesiya bark, 27 Jun. 2010, S.J.Taerum, CMW41662 = CBS139659.

Host.

Pinus kesiya.

Beetle vector.

Tomicus brevipilosus.

Distribution.

At present known only from Yunnan, China.

Notes.

The synnematous asexual state of O. brevipilosi corresponds with similar structures of O. brunneo-ciliatum as described by Linnakoski et al. (2016). The hyalorhinocladiella state resembles those of O. brunneolum, O. macroclavatum, O. pseudocatenulatum (Linnakoski et al. 2016) and O. poligraphi (Yin et al. 2016). However, the morphology of these structures is not sufficient to distinguish between the species in the complex, and DNA sequences of the BT and EF gene regions are recommended for accurate species identification.

 LinnakoskiRJankowiakRVillariCKirisitsTSolheimHDe BeerZWWingfieldMJ (2016) The Ophiostoma clavatum species complex: a newly defined group in the Ophiostomatales including three novel taxa. Antonie van Leeuwenhoek 109: 987–1018. https://doi.org/10.1007/s10482-016-0700-y YinMLWingfieldMJZhouXDDe BeerZW (2016) Multigene phylogenies and morphological characterization of five new Ophiostoma spp. associated with spruce-infesting bark beetles in China. Fungal Biology 120: 454–470. http://dx.doi.org/10.1016/j.funbio.2015.12.004