This PDF file includes:

• fig. S1. No change in rRNA levels during aging.
  
• fig. S2. Cohesin occupancy is not affected by NTS1 transcription, but cohesins are reduced during aging.
  
• fig. S3. Appearance of two major chromosomal bands containing rDNA during aging.
  
• fig. S4. The two major chromosomal bands containing rDNA appear to comprise mainly rDNA repeats in old cells.
  
• fig. S5. Increased chromosomal instability proportional to chromosome length and rDNA instability is observed during aging in WT cells.
  
• fig. S6. The DNA damage checkpoint is intact in old cells.
  
• fig. S7. Rad51 and Mre11 overexpression.
  
• fig. S8. Model for rDNA instability and reduced HR causing global genomic instability to limit replicative life span.
  
• table S1. Yeast strains used in this study.
  
• table S2. Primers used in this study.
  
• References (5, 19, 53)

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