Oral Application of Recombinant Bacillus subtilis Spores to Dogs Results in a Humoral Response against Specific Echinococcus granulosus Paramyosin and Tropomyosin Antigens

Supplemental material

• Supplemental file 1 -

  Fig. S1. ELISA for the detection of specific immune response against H₆-mCherry and H₆-TasA in sera from dogs #1 and #2 immunized with spores of B. subtilis tasA/sinR/lux/TasA-mCherry strain at increasing times postapplication. Fig. S2. Detection of humoral IgA response. Fig. S3. Specific humoral immune response of dogs after oral application of recombinant Bacillus subtilis spore carrying Echinococcus granulosus antigens. Table S1. Primers used for plasmid construction. Table S2. Assignment of the 16S rrnE gene of bacteria isolated from dogs' feces.

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