Fig. S1. siRNA-mediated downregulation of cGas expression reduces Ifnb induction in response to S. pneumoniae and pneumococcal DNA in BMDMs. Fig. S2. The cGAS-STING pathway has little effect on the expression of proinflammatory cytokine mRNA during S. pneumoniae infection. Fig. S3. The HAQ TMEM173 allele has an impaired capacity for cytokine production in response to S. pneumoniae infection or bacterial DNA stimulation. Fig. S4. Weak cGAS-dependent induction of type I IFNs in THP-1 cells after infection with S. pneumoniae or stimulation with DNA or cGAMP. Fig. S5. Cells from R232H TMEM173 homozygous individuals produce similar levels of proinflammatory cytokines as WT STING-expressing cells following stimulation with bacterial DNA or S. pneumoniae infection.
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