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[]
["List of antibodies and dilutions used in the study"]
[]
[]
[]
["Primary antibodies used"]
[]
["Antibody","Raised in ","Application","Dilution ","Source","Catalogue number"]
["METTL3","Rabbit","Western Blot","1:3000","Proteintech Group","15073-1-AP"]
["METTL14","Rabbit","Western Blot","1:3000","Sigma","HPA038002"]
["WTAP","Mouse","Western Blot","1:1000","Proteintech Group","60188-1-lg"]
["VIRMA","Rabbit","Western Blot","1:1000","Bethyl Lab","A302-124A"]
["ZC3H13","Rabbit","Western Blot","1:1000","Bethyl Lab","A300-748A"]
["HAKAI/CBLL1","Rabbit","Western Blot","1:1000","Bethyl Lab","A302-969A"]
["TRIM28/KAP-1","Rabbit","Western Blot","1:1000","Bethyl Lab","A300-274A"]
["HNRNPH","Rabbit","Western Blot","1:1000","Bethyl Lab","A300-511A"]
["HNRNPK","Rabbit","Western Blot","1:1000","Bethyl Lab","A300-678A"]
["HNRNPU","Rabbit","Western Blot","1:1000","Bethyl Lab","A300-689A"]
["CPSF5","Rabbit","Western Blot","1:1000","Proteintech Group","10322-1-AP"]
["CPSF6","Rabbit","Western Blot","1:1000","Bethyl Lab","A301-356A-M"]
["CSTF2T","Rabbit","Western Blot","1:1000","Bethyl Lab","A301-487A-M"]
["POLR2B","Rabbit","Western Blot","1:1000","Proteintech Group","20370-1-AP"]
["WTAP","Mouse","Immunofluorescence","1:150","Proteintech Group","60188-1-lg"]
["VIRMA","Rabbit","Immunofluorescence","1:50","Bethyl Lab","A302-124A"]
[]
["Secondary antibodies used"]
["Antibody","Raised in ",null,"Dilution ","Source","Catalogue number"]
["anti-GAPDH(HRP conjugate)","Goat","Western Blot","1:5000","GeneScript","A00192"]
["anti-Mabbit","Rabbit","Western Blot","1:10000","Santa Cruz","SC2357"]
["Alexa488 anti-rabbit(H+L)","Donkey","Immunofluorescence","1:300","Molecular Probes","A11034"]
["Alexa647 anti-muuse(H+L)","Goat","Immunofluorescence","1:300","Molecular Probes","A21236"]
["anti-flag(HRP conjugate)","Mouse","Western Blot","1:5000","Sigma","A8592"]
["anti-HA(HRP conjugate)",null,"Western Blot","1:1000","Cell Signaling",14031]
[]
["antibodies used for Immunoprecipitation"]
[]
["Antibody","Raised in ","Dilution ","Source","Catalogue number"]
["anti-m6A","Rabbit","1ug/ug mRNA","Synaptic Systems",202003]
["VIRMA","Rabbit","5ug/mg cell lysate","Bethyl Lab","A302-124A"]