Genomic profiling of short- and long-term caloric restriction effects in the liver of aging mice -- Data Supplement - Supporting Information -- Proceedings of the National Academy of Sciences

Supporting information for Cao et al. (September 4, 2001) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.191313598.

Fig. 4.
Validation of array data with Northern blots. The expression of eight genes was analyzed by Northern blotting. Shown are the results for young and old, control, and LT-CR mice. RNA samples from six mice, including the three used for the microarray studies, were analyzed from each experimental group. The bars representing the Northern blot data are the ratio of the means of each mRNA level measured for young LT-CR and control mice, and old LT-CR and control mice. The bars representing the microarray data are the average fold change in the specific mRNA for LT-CR and control mice in each age group. The genes analyzed were glucose-6-phosphatase (G6pc), heat shock cognate protein 70 (Hsc70), CCAAT/enhancer-binding protein b (Cebpb), glucose-regulated protein 78 kDa (Hspa5), calcium-binding protein (Cai), pyruvate dehydrogenase E1a subunit (Pdha1), glucose-regulated protein 58 kDa (Grp58), and regucalcin (Rgn).