Naive CD4 T cells inhibit CD28-costimulated R5 HIV replication in memory CD4 T cells -- Data Supplement - Supporting Figure 6 -- Proceedings of the National Academy of Sciences

Supporting information for Mengozzi et al. (September 18, 2001) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.21120509.

Supporting Figure 6 Fig. 6.
M2- or naïve-mediated suppression of M1 HIV replication is not caused by inhibition of M1 cell division. Cells were sorted and infected as described in Figs. 1 and 2. M1 cells were stained with carboxy fluorescein succinimethyl (CFSE) and cultured either unstimulated (Upper left) or in the presence of anti-CD3/CD28 beads (3 beads/cell, Right). The histograms of CFSE expression at day 5 are shown. Unstimulated M1 cells (Upper Left) show uniform retention of CFSE, whereas stimulated M1 cells (Upper Right) show a series of peaks exhibiting serial halving of CFSE fluorescence, caused by cell division. The mean fluorescence intensities for the six CFSE peaks from right to left are 294, 153, 77, 39, 20, and 10, respectively. The gray peak represents the autofluorescence of anti-CD3/CD28 bead-stimulated unstained M1 cells. (Lower Right) CFSE-stained M1 cells were cocultured with unstained M2 or naïve, or M1 cells as a control, as described in Fig. 3. The histograms of CFSE fluorescence in cocultures 5 days after stimulation are shown and represent M1 cell division in cocultures with M1 (dotted gray line, control), with M2 (thin black line) or naïve cells (solid black line). The identical gates as for Upper Right were applied. Analysis of the data in terms of average number of M1 cell divisions (division index, as described in supporting Methods) showed no difference in M1 proliferation rate in the different coculture conditions. Data are representative of two independent experiments.