This PDF file includes:

• fig. S1. Fabrication, operation, and setup of the SurfaceChIP microfluidic device.
  
• fig. S2. Data quality of SurfaceChIP-seq.
  
• fig. S3. Reproducibility of SurfaceChIP-seq data by eight-channel devices produced in different batches.
  
• fig. S4. SurfaceChIP-seq with AGP linker.
  
• fig. S5. Rank of H3K4me3, H3K27ac, and H3K27me3 signals at promoters plotted against mRNA rank (generated by mRNA-seq).
  
• Legends for tables S1 to S5

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Other Supplementary Material for this manuscript includes the following:

• table S1 (Microsoft Excel format). Metadata of SurfaceChIP-seq and mRNA-seq.
  
• table S2 (Microsoft Excel format). A list of genes with differential H3K4me3 mark at promoters across PNeuN+ and CNeuN+ populations.
  
• table S3 (Microsoft Excel format). A list of genes with differential H3K27ac mark at promoters across PNeuN+ and CNeuN+ populations.
  
• table S4 (Microsoft Excel format). A list of genes with differential H3K27me3 mark at promoters across PNeuN+ and CNeuN+ populations.
  
• table S5 (Microsoft Excel format). A list of differentially expressed genes (fold change > 4; P < 0.05, t test) with correlated differential histone modification in H3K4me3 or H3K27me3 at promoters across PNeuN+ and CNeuN+ populations.