This PDF file includes:

• fig. S1. Multiwindow chips compatible with commercial LC-TEM holders.
  
• fig. S2. Additional experimental data from Fig. 2.
• fig. S3. Imaging path and thickness of multiwindow devices for no flow versus flow.
  
• fig. S4. Low-dose imaging of biological samples.
  
• fig. S5. Improved sampling of nonuniform samples with multiwindow areas.
  
• fig. S6. Illustration of microfabrication workflow.
  
• Supplementary protocol for fabrication of multiwindow devices
  
• Legend for movie S1

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Other Supplementary Material for this manuscript includes the following:

• movie S1 (.mp4 format). Demonstration of in situ liquid cell “low-dose” imaging.