Peptide Level Turnover Measurements Enable the Study of Proteoform Dynamics

Supplemental Data

• Supplementary Figures - Supplementary Figures
• Supplementary Methods - More detailed description of applied methods for data normalization, curve fitting, calculation of protein copy numbers and setup of the PRM assay
• Supplementary Table S1 - TMT intensities, ratios and curve fitting parameters for the four independent cell culture experiments
• Supplementary Table S2 - Protein data of the MS1 (pulsed SILAC) and the MS3 (pulsed SILAC-TMT) quantification approach
• Supplementary Table S3 - Proteins annotated with determined turnover, biophysical properties and functional categories
• Supplementary Table S4 - Inclusion list and results of the PRM experiment screening for increased turnover of respiratory chain complex I members upon induction of oxidative stress
• Supplementary Table S5 - Results of the statistical testing of differences between single peptide and whole protein turnover