Cysteamine, an Endogenous Aminothiol, and Cystamine, the Disulfide Product of Oxidation, Increase Pseudomonas aeruginosa Sensitivity to Reactive Oxygen and Nitrogen Species and Potentiate Therapeutic Antibiotics against Bacterial Infection

Supplemental material

• Supplemental file 1 -

  Fig. S1. Relative H2DCFDA fluorescence of Pseudomonas aeruginosa PAO1 cultures treated with different combinations of ciprofloxacin and cystamine as shown over time compared to background fluorescence in media from which data at 2 h is analyzed as shown in Fig. 2. Fig. S2. Addition of sub-MIC concentrations of CYS to culture media reduces the production of phenazine pigments in a range of type and clinical strains of P. aeruginosa without affecting growth. Fig. S3. Addition of sub-MIC concentrations of CYS to culture media reduces the release of pyomelanin pigment from these B. cenocepacia strains into the agar.

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