This PDF file includes:

• fig. S1. Cotranslational folding of HaloTag can be measured using FP.
  
• fig. S2. Addition of the peptidyl-proline isomerase CypA does not affect HaloTag refolding or cotranslational folding rates.
  
• fig. S3. Aggregation of HaloTag.
  
• fig. S4. Cysteine accessibility of WT HaloTag.
  
• fig. S5. Characterization of Halo* cysteine mutants.
  
• fig. S6. Gels for data shown in Fig. 4.
  
• fig. S7. Characterization of Halo* E121C cysteine accessibility.
  
• table S1. Kinetic data obtained for HaloTag folding using FP.
  
• table S2. Determination of HaloTag folding efficiency under different conditions.
  
• table S3. Folding rates of HaloTag and variants measured by FP.
  
• Legend for table S4

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Other Supplementary Material for this manuscript includes the following:

• table S4 (Microsoft Excel format). Normalized HX-MS data.