Identification of a conserved erythroid specific domain of histone acetylation across the alpha -globin gene cluster -- Data Supplement - Supporting Information -- Proceedings of the National Academy of Sciences

Supporting information for Anguita et al. (October 2, 2001) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.201413098.

Table 1.
Coordinates for the probes from human chromosome 16p, mouse chromosome 11, and chicken a cluster

Probe	Coordinate	Forward primer	Reverse primer	Ref.	Other
Human
h1	45812-47175	CTGAAGAAGGCCATCCAGAGAT	CAGTGATGAAGAGGGTTGTCCTG
h2	53150-55900	__________	__________	Nicholls, R. D. Fischel-Ghodsian, N. & Higgs, D. R. (1987) Cell 49, 369-378
h3	66093-66805	TCACCCCAGCACCAATACTAGC	GCCCTGGATGTGATTCCACAC
h4	71545-72285	__________	__________	Jarman, A. P. & Higgs, D. R. (1988) Am. J. Hum. Genet. 43, 249-256
h5	78700-78900	__________	__________		cDNA from exon 13 of the C16orf35 gene
h6	82646-83312	AAGGAGAACTTGGCAAGAACGG	TGCAGCTCATCTGGTGTACTGG
h7	91835-92895	CATCGGTCTCCACTCAAGTGT	CCAAAGAGAGGTAAGGTTCTG
h8	103385-103907	GAAAACCAGCAGGCTCCAGG	TGTAAGTCCATCCAGGTGTGAGTTC
h9	109763-110350	TCCTTCCTCATAGCATGGCTGC	GACCAGGTTTGCACCCTAGAATG
h10	120327-120892	CCCAAATTATTCAGCAAGCCC	AACTCTTCATTTCCCCTGCTCC
h11	133165-133635	TTATCGGTCCTGCCACTTGGTCTG	ATGCTGGTGTCCACTGGTGGTTTC
h12	138724-139207	TAAGGACCATTCCTCAGCGTGG	GCTCATCTGGGGCTTCTTTCAG
h13a	143850-145330	__________	__________	Nicholls, R. D. et al. (1987) Cell 49, 369-378
h14	149845-150992	__________	__________	Goodbourn, S. E. Y., Higgs, D. R., Clegg, J. B. & Weatherall, D. J. (1983) Proc. Natl. Acad. Sci. USA 80, 5022-5026
h13b	154480-156100	__________	__________	Nicholls, R. D. et al. (1987) Cell 49, 369-378
h15a	162300-163800	__________	__________	Lauer, J., Shen, C.-K. J. & Maniatis, T. (1980) Cell 20, 119-130
h15b	166100-167600	__________	__________	Lauer, J. et al. (1980) Cell 20, 119-130
h16	170300-171130	__________	__________	Nicholls, R. D. et al. (1987) Cell 49, 369-378
h17	173071-173363	CTCACCTCAATCATCCTGTGTGC	TTGGATCTGCACCTCTGGGTAG
h18	175999-177279	__________	__________	Jarman, A. P., Nicholls, R. D., Weatherall, D. J., Clegg, J. B. & Higgs, D. R. (1986) EMBO J. 5, 1857-1863
h19	184678-185118	GGACCACAAATCCTCTGCAACTG	AAGGTGTCCATCCATGTGTGCC
h20	194809-195111	TGGGAGCATTTTCAGCATCACTAG	AGGTGCAGTATTAAATCAGGGCTG
h21	199516-199967	CACTTTTCCGACTGTCAGCCAG	TCTGGGAGTCAGCCTTCATCAG
h22	258000-258800	__________	__________	Flint, J., Thomas, K., Micklem, G., Raynham, H., Clark, K., Doggett, N. A., King, A. & Higgs, D. R. (1997) Nat. Genet. 15, 252-257
Mouse
m1	1-1620	__________	__________	Flint, J., Tufarelli, C., Peden, J., Clark, K., Daniels, R. J., Hardison, R., Miller, W., Philipsen, S., Tan-Un, K. C., McMorrow, T., Frampton, J., Alter, B., Frischauf, A.-M. & Higgs, D. R. (2001) Hum. Mol. Genet. 10, 371-382	Dr. C. Tufarelli gift
m2	52600-53670	CACACACCCAGACCGATCATTG	TGAGTCTTGAGGCTCCCCAAAC
m3	55112-56253	TATTCTGTCCCCTTACCCCAATC	CATAACCTACACTCCCAGGCTTGTC
m4	82759-83564	TCTTGGTCGCTCTCCTTACAGC	GTTATCTTTCCCGCACTGGATG
m5	90505-91815	CCCACTGTGCTATGACTGTAGAG	CATTATAGGGGTAAGCTACCATACC
m6	95915-96763	ACATCTTTGACCCCAGTCCCTC	AACTGCATGTTGACCATAGCCAG
m7	99579-100162	TATCACCCAGCAGATCCC	GCCTGCCTTAGCCTGGCT	Zhao, Q. Z., Liang, X. L., Mitra, S., Gourdon, G. & Alter, B. P. (1996) Mamm. Genom 7, 749-153
m8a	123320-124623	CTAACTTCTTCCCAAACTGCCATC	GATAAGGACCCTCCAAAGACGATC
8b	136224-137527	CTAACTTCTTCCCAAACTGCCATC	GATAAGGACCCTCCAAAGACGATC
m9	141886-142293	TTCTGCTGAGGTCTGAGATGGG	GCTCCATTCTTCATCACTGCATG
m10	150136-150896	__________	__________		761 bp HindIII fragment, Dr. C. Tufarelli gift
m11	152427-153517	CTCTCTTTGCACTTTATG	GAGAGAACGGTCACTTG
Chicken
c1	73-473	TCAAGCAGAGGACAAGCAGTGG	GAAACCTGCCTCACTCCTGATG
c2	2726-3250	ACAGTCCACAGCTTGATGTCACC	GGATGGTCTATTTTCAGCATGGC
c3	10609-11051	ACAATAACCACATGCACTCCGAC	AATGAGGTGGGTGATAAGGAAGC
c4	18444-18933	CCTGCACATGAGTCACATCAGG	CATGGTGGCAAGCTAGGAGTGTAG
c5	20897-21479	TGGGAGATGCCTAATGAGACTAGG	TAGCTGACCAAAGGACACTGAATG
c6	23912-24795	CAGTGAATTAATGTCAGC	GCTGAAGATGATTACTTTG
c7	33579-34088	CAAGTGCCTGCTTGCTGCTAAG	ATGTGCAGTGGTTTTGAGGG
c8	46805-47390	GCTCACAGCAGTTTGAAGACCTTG	CCCAGCCAGGTAACAGCTTAAAC
c9	52901-53249	CAAGAACAACGTCAAGGGCATC	GTCATCAATGTGGTTGGCAGC
c10	54038-54587	ACATTTCCCAGCGTCTGTCATC	GGCTTTCTCAAACCTCCATCTCTG
c11	56265-56787	TCCTAAATGTCAGCCTGAACTGC	GGTAGATAGATCCCGAAAGGAGTGG
c12	62913-63443	TGTTGCCTGTAGGAGCCCATAC	ATTCATGCCCCAGAGCAGGATG
c13	65692-66114	CGTGTGTTTCCATCCCTTGG	TCCAGAACAGGCATATCAGTGAGTC
c14	78234-78743	GCTCATTCTTCTCTGTTCAGGGTG	GGGTCTTCCAGGTGAGTAACATGC

The primers from the probes generated by PCR (all defined in the 5' to 3' orientation) are indicated. The other probes were previously described (see references), or its origin is indicated.