Oligomerization of the integrin alpha IIbbeta 3: Roles of the transmembrane and cytoplasmic domains -- Data Supplement - Supporting Figure 7 -- Proceedings of the National Academy of Sciences

Li et al. (October 16, 2001) Proc. Natl. Acad. Sci. USA, 10.1073/pnas.221463098.

Supporting Figure 7
Fig. 7.
Expression and purification of aIIb and b3 TM-CYTO proteins, shown on an SDS polyacrylamide gel. Lane 1: Molecular mass markers [sizes labeled (left) in kDa]. Lane 2: Whole cell lysate for glutathione S-transferase (GST)-b3 fusion protein. Lane 3: GST-b3 fusion protein eluted from the glutathione-sepharose column. Lane 4: Thrombin cleaves GST-b3to GST (top band) and b3 TM-CYTO protein in the presence of N-octyl glucoside. Lane 5: Pure b3TM-CYTO protein. Lane 6: Whole cell lysate for GST-aIIb fusion protein. Lane 7: GST-aIIb fusion protein eluted from the glutathione-sepharose column. Lane 8: Thrombin cleaves GST-a IIbto GST and a IIb TM-CYTO protein in the presence of N-octyl glucoside (adoes not stain as well as b protein). Lane 9: Pure aIIbTM-CYTO protein (overloaded).