This PDF file includes:

• Supplementary Materials and Methods
• fig. S1. Comparison of homozygous Arc-PBS mouse to WT mouse.
• fig. S2. PCP binding to PBS-tagged mRNAs does not alter mRNA integrity.
• fig. S3. Persistent synchronized Ca²⁺ spikes after bicuculline washout.
• fig. S4. Synchronized Ca²⁺ spikes during bicuculline incubation.
• fig. S5. Plot of Ca²⁺ activity and Arc transcription dynamics.
• fig. S6. Comparison of somatic Ca²⁺ spikes between neurons with and without Arc transcription within 30 min after bicuculline stimulation.
• table S1. Sequence of the probes used for smFISH against the Arc-CDS and PBS linkers (PBS).
• Legends for movies S1 to S4
• Reference (61)

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Other Supplementary Material for this manuscript includes the following:

• movie S1 (.mp4 format). Immediate early activation of transcription from PBS-tagged Arc allele.
• movie S2 (.mp4 format). Ca²⁺ activity of neurons induced by bicuculline stimulation.
• movie S3 (.mp4 format). Arc transcription dynamics induced by bicuculline stimulation.
• movie S4 (.mp4 format). Dendritic transport of Arc mRNA.