Proteins Encoded by the gerP Operon Are Localized to the Inner Coat in Bacillus cereus Spores and Are Dependent on GerPA and SafA for Assembly

Supplemental material

  • Supplemental file 1 -

    Amino acid sequence identity of B. cereus 14579 GerP proteins with orthologues present in other Bacillus species (Table S1); amino acid sequence identity between predicted B. cereus 14579 GerPF orthologues (Table S2); agarose gel showing the products of diagnostic PCRs used to characterize the various B. cereus GerP null mutant strains (Fig. S1); thin-section TEM micrographs of spores of B. cereus 14579 wild-type and ΔgerP strains (Fig. S2); germination of B. cereus 14579 wild-type and gerP null spores in the presence of l-alanine (Fig. S3) or nutrient and nonnutrient germinant compounds (Fig. S4); phase-contrast and fluorescence microscopy analysis of sporulating B. cereus gerPB (Fig. S5) or gerP null (Fig. S6) cells complemented with plasmid-borne copies of gerPA-gfp, gerPB-gfp, gerPC-gfp, gerPD-gfp, gerPE-gfp, or gerPF-gfp.

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