Detection of “Top 7” STEC in calves on farms (Table S1); logistic mixed-effects regression model of factors associated with prevalence of any STEC O26 (Table S2), STEC O103 (Table S3), or STEC O145 (Table S4); sample size calculations for farms and calves with cluster-sample design effect (Table S5); New Zealand dairy farms per region sampled and milking herd size (Table S6); sensitivity (Table S7) and specificity (Table S8) of NeoSEEK and reverse transcriptase PCR assays for detection of “Top 7” STEC serogroups in calf fecal enrichment samples; BioProject PRJNA396667 metadata from raw sequence data used in this study (Table S9); location, animal, pen, and farm management factors evaluated against outcome variables of “Top 7” STEC prevalence in dairy calves (Table S10); heatmap of SNP distances between E. coli serogroup O26 isolates (Fig. S1).
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