Mutation of neutral variants chosen at random in generation 3 (Fig. S1); thermostability and initial rates of neutral variants from generation 3 (Fig. S2); SDS-PAGE of PaDa-I and neutral variants from culture broth (Fig. S3); purification of UPO variants (Fig. S4); thermostability and activity in the presence of organic cosolvents (Fig. S5); amino acid mutations of neutral clones from generation 8 (Table S1).
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