Unravelling the Reduction Pathway as an Alternative Metabolic Route to Hydroxycinnamate Decarboxylation in Lactobacillus plantarum

Supplemental material

  • Supplemental file 1 -

    Effect of disruption of lp_1422 (hcrR), lp_1424 (hcrA), lp_1425 (hcrB), and lp_1426 (hcrC) on m-coumarate reduction in L. plantarum WCFS1 (Fig. S1); purification and enzymatic activity of E. coli extracts expressing L. plantarum HcrA and recombinant HcrA protein (Fig. S2); schematic representation of cinnamic acid and the hydroxycinnamic acids assayed in this study (Fig. S3); schematic representation of the modular structures of HcrA and HcrB proteins (Fig. S4); comparison of amino acid sequences of putative HcrA (Fig. S5) and HcrB (Fig. S6) proteins from lactic acid bacteria.

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