Heterologous Expression of Mycobacterium Alkene Monooxygenases in Gram-Positive and Gram-Negative Bacterial Hosts

Supplemental material

• Supplemental file 1 -

  Structure of plasmid pUS116 (Fig. S1); alignment of synthetic vs. native etnHABCD sequences (Fig. S2); evidence of ethene MO expression in SDS-PAGE of M. smegmatis mc²-155 cell extracts (Fig. S3); structure modeling of EtnH of strain NBB4 using SWISS-MODEL (Fig. S4); SDS-PAGE comparison of soluble and total cell fractions of E. coli BL21 cells expressing etnHABCD genes (Fig. S5); purification of His-tagged EtnB from BL21(DE3) cells (Fig. S6).

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