Plasmodium falciparum MSP3 Exists in a Complex on the Merozoite Surface and Generates Antibody Response during Natural Infection

Supplemental material

  • Supplemental file 1 -

    Fig. S1. Expression and purification of MSP3N antigen. Fig. S2. Schematic and expression of P. falciparum merozoite proteins. Fig. S3. Immunofluorescence assay with P. falciparum 3D7 parasites using anti-human rMSP3N antibodies. Fig. S4. SPR Profile of non-MSP3N binding proteins. Table S1. List of primer sequences. Table S2. Showing, ANOVA: single-factor analysis on seropositive data of three groups of populations, Danes, Indians, and Liberians. Table S3A. Showing, ANOVA: single-factor analysis on opsonic phagocytosis assay data. Table S3B. Showing standard mean error and confidence (CI) on opsonic phagocytosis assay data. Table S4A. Showing, ANOVA: single-factor analysis on GIA experiment data obtained from anti-MSP3N human MSP3 antibodies. Table S4B. Showing, standard error of the means of GIA experiment data obtained from anti-MSP3 human antibodies. Table S5A. Showing, ANOVA: single-factor analysis of GIA data obtained from anti-MSP3N rabbit total IgGs. Table S5B. Showing, standard error of the means of GIA experiment data obtained from anti-MSP3N rabbit total IgGs.

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  • Supplemental file 2 -

    Data Set 1a. Immunopulldown LC/MS data table generated using anti-rMSP3N human antibodies on P. falciparum merozoite lyzate.

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  • Supplemental file 3 -

    Data Set 1b. Full list of proteins identified in LC-MS/MS analysis for immunoprecipitation with preimmune rabbit IgGs.

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  • Supplemental file 4 -

    Data Set 4b. Experiment 4b of immunopulldown LC/MS data.

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  • Supplemental file 5 -

    Data Set 3b. Experiment 3b of immunopulldown LC/MS data.

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  • Supplemental file 6 -

    Data Set 2b. Experiment 2b of immunopulldown LC/MS data.

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  • Supplemental file 7 -

    Data Set 2a. Experiment 2 of immunopulldown LC/MS data.

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  • Supplemental file 8 -

    Data Set 3a. Experiment 3a of immunopulldown LC/MS data.

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  • Supplemental file 9 -

    Data Set 4a. Experiment 4a of immunopulldown LC/MS data.

    XLSX, 161K