RpoN-Dependent Direct Regulation of Quorum Sensing and the Type VI Secretion System in Pseudomonas aeruginosa PAO1

Supplemental material

• Supplemental file 1 -

  Fig. S1 (pAK1900-rpoN complementation of the phenotypes of P. aeruginosa), S2 (SDS-PAGE of RpoN and RpoF after Ni-NTA column affinity chromatography purification), S3 (RpoN and expression of lasR), S4 (RpoN-binding motifs), S5 to S8 (Mapping of the RpoN motif in the promoters of pqsA [S5], pqsR [S6], hcpA [S7], and hcpB [S8]), S9 (Check for insertions at attTn7), S10 (Comparison of ChIP-seq and RNA-seq between PAO1 and other strains), and S11 (RpoN effect on expression of downstream genes in mid-log and stationary phases) and Tables S1 (RpoN-dependent EBPs), S2 (Strains and plasmids), and S3 (Primers)

  PDF, 1.3M

• Supplemental file 2 -

  Table S4 (Whole-genome location analysis of RpoN from ChIP-seq in PAO1)

  XLSX, 153K

• Supplemental file 3 -

  Table S5 (Whole-genome location analysis of RpoN from RNA-seq in PAO1)

  XLSX, 643K